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Analysis of two precipitation methods on the yield, structural features and activity of sulfated polysaccharides from Gracilaria cornea (Rhodophyta) Biological Sciences
Souza, Ricardo Basto; Queiroz, Ismael Nilo Lino de; Silva Neto, Antônio Alves da; Carneiro, José Gerardo; Rodrigues, José Ariévilo Gurgel; Benevides, Norma Maria Barros.
The global demand for natural products from seaweeds has increased worldwide; however, no description of the use of isoamly alcohol (IAA) for obtaining of sulfated polysaccharides (SPs) has been reported. We investigated the efficiency of two precipitation methods (M) in obtaining SPs from the red seaweed Gracilaria cornea. SPs enzymatically isolated were concentrated with cetylpyridinium chloride (M I) or IAA (M II) and extracts were examined with regard to their yield, structural features and in vitro effects on the activated partial thromboplastin time (APTT) using normal human plasma and standard heparin (193 IU mg-1). Yield difference reached 12.99%. Quantitative determination of sulfate was similar between the two methods (̴ 26%), but extracts...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhodophyceae; Polysulfated; Precipitation methods; Structural analysis; APTT test.
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/24016
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